一氧化碳对肿瘤坏死因子-α和白细胞介素-1β共同诱导的人牙龈成纤维细胞黏附分子表达的影响

[摘要] 目的 研究一氧化碳对炎性环境下人牙龈成纤维细胞黏附分子表达的影响。方法 以50 ng·mL-1的肿瘤坏死因子（TNF）-α和10 ng·mL-1的白细胞介素（IL）-1β刺激加入或不加入500 μmol·L-1一氧化碳释放分子（CORM）的人牙龈成纤维细胞，用Western blot法检测细胞间黏附分子（ICAM）-1、血管细胞黏附分子（VCAM）-1的蛋白表达，用逆转录多聚酶链反应（RT-PCR）检测黏附分子的mRNA表达，用瞬时转染和报告基因测定法分析NF-κB的活性。结果 TNF-α和IL-1β共同刺激后，人牙龈成纤维细胞ICAM-1和VCAM-1的mRNA表达和蛋白表达均显著增强。CORM的加入可显著抑制ICAM-1和VCAM-1的表达。CORM可显著降低ICAM-1和VCAM-1诱导的NF-κB的活性。结论 一氧化碳有可能成为牙周病治疗的一种极具潜力的新物质。

[关键词] 人牙龈成纤维细胞； 牙周炎； 黏附分子； 一氧化碳

[中图分类号] R 781.4 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.03.020

Influence of carbon monoxide on the expression of adhesion molecules stimulated with tumor necrosis factor-α and interleukin-1β on human gingival fibroblasts Zhao Huaqiang1，2, Wei Lingling1，3, Hou Meng1，3, Mu Pingping1，3, Wei Fengcai4, Song Hui1，3, Yang Pishan1，3. （1. Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China; 2. Dept. of Oral and Maxillofacial Surgery, Stomatology Hospital of Shandong University, Jinan 250012, China; 3. Dept. of Periodontology, Stomatology Hospital of Shandong University, Jinan 250012, China; 4. Dept. of Stomatology, Qilu Hospital of Shandong University, Jinan 250012, China）

[Abstract] Objective To investigate the influence of carbon monoxide on the expression of adhesion molecules stimulated by inflammatory cytokines on human gingival fibroblasts. Methods Human gingival fibroblasts were stimu-lated with 50 ng·mL-1 tumor necrosis factor（TNF）-α and 10 ng·mL-1 interleukin（IL）-1β concurrently in the presence

or absence of 500 μmol·L-1 carbon monoxide releasing molecule（CORM）. Expression of intercellular adhesion molecule

（ICAM）-1, vascular cell adhesion molecule（VCAM）-1 at protein and mRNA level was examined by Western blot and

reverse transcription polymerase chain reaction（RT-PCR）, respectively. Activity of transcription factor NF-κB was eva-

luated by reporter gene assay. Results Expression of ICAM-1 and VCAM-1 on human gingival fibroblasts increased dramatically after concurrent stimulation of TNF-α and IL-1β, while CORM inhibited the upregulation of ICAM-1 and VCAM-1. CORM decreased the activity of NF-κB stimulated by TNF-α and IL-1β. Conclusion Carbon monoxide could be a promising way in treating of periodontitis.

[Key words] human gingival fibroblasts； periodontitis； adhesion molecules； carbon monoxide

龈下菌斑中黏附的牙周致病菌引发的慢性炎症是牙周炎致病过程中牙周组织破坏的主要原因。牙周炎的主要病理特点是病变区域中大量免疫活性细胞的迁移和浸润。这些浸润的免疫活性细胞可分泌大量的炎性因子，如肿瘤坏死因子（tumor necrosis fac-tor，TNF）-α、白细胞介素（interleukin，IL）-1β、IL-